Fig. 6.
Tyrosine phosphorylation of pp125FAK.
Nonadherent or adherent 293 cells stably expressing wild-type αIIbβ3 (lanes 1 and 3) or Tyr143HisαIIbβ3 (lanes 2 and 4) on fibrinogen were lysed in Triton X-100 buffer containing sodium vanadate. pp125FAK was immunoprecipitated with 1 μg rabbit polyclonal antibody specific for FAK. Precipitates were separated on 7.5% SDS-PAGE and transferred to a PVDF membrane. Phosphotyrosine was detected with monoclonal antibody 4G10. To monitor the loading of gel lanes, the blots were reprobed with anti-FAK.
