Fig. 7.
![Fig. 7. Fibrin clot retraction mediated by the cells stably expressing mutant αIIbβ3. / 293 cells (control), 293 cells stably expressing wild-type β3 alone, wild-type αIIbβ3, Tyr143HisαIIbβ3, or KO mutant αIIbβ3 suspended in Tyrode/HEPES buffer (2 × 106 cells/mL) were incubated with 10 mM tranexamic acid, 250 μg/mL fibrinogen, and 2 mM CaCl2 at 37°C in a siliconized glass tube in the presence of 50 μM cyclo(RGDfV) (αvβ3-specific antagonist). Then 1 U thrombin was added to 1 mL cell suspension. Clot retraction was monitored by digital photography every 30 minutes. Data were expressed as % clot retraction = [(area t0 − areat)/area t0] × 100. Results are representative of 3 separate experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/9/10.1182_blood-2002-07-2144/4/h80934230007.jpeg?Expires=1735817669&Signature=J02LTRsF16ZAXkmkPx~socZLeTku1a7OZAf7rOgQGmKO2cbzmZFkLUgkFI4YjLq7CmHu7vJZvMT2RF9azNbwdyTpMtY9bSheNO5y2h-vaOf~8qbt6Pd-ccpJwJAwIg1VgX-7236bVfnkr7vL4JXjYPudm38IRJ-05EtjVJ9esDFtGxY6lbU-3iZ1pE3HUclfoUJB0ff7cMvOK5ity2L5CU4rrZPhwb1jvViOq9FB5EcmmpDOhqWUBpy7DejM2JCbjdPCs7DC8E3sIDFcM4fjj3MfOKokhCX80nQvsGtVcX7zkyrlxaKBNmDTKLt--qQDhNDQykjRy4LtnHz-AYetBg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Fibrin clot retraction mediated by the cells stably expressing mutant αIIbβ3.
293 cells (control), 293 cells stably expressing wild-type β3 alone, wild-type αIIbβ3, Tyr143HisαIIbβ3, or KO mutant αIIbβ3 suspended in Tyrode/HEPES buffer (2 × 106 cells/mL) were incubated with 10 mM tranexamic acid, 250 μg/mL fibrinogen, and 2 mM CaCl2 at 37°C in a siliconized glass tube in the presence of 50 μM cyclo(RGDfV) (αvβ3-specific antagonist). Then 1 U thrombin was added to 1 mL cell suspension. Clot retraction was monitored by digital photography every 30 minutes. Data were expressed as % clot retraction = [(area t0 − areat)/area t0] × 100. Results are representative of 3 separate experiments.
