ALK-1QD inhibits HMVEC-d migration in a wound assay.

(A) At 15 hours after infection, the cell monolayer was scratched to create a wound. At time 0, 24, and 48 hours after wounding, the cells were observed by phase contrast microscopy and photographed. Original magnification, × 50. (B) Comparison of the path of migration of AdALK-1QD and Adβ-gal HMVEC-d infected cells at their initial positions (1-6, time 0, wounding) and at their final positions (1′-6′, 48 hours after wounding). (C) The mean wound width was measured at time 0 (wounding) and 48 hours after wounding (n = 10). D: Infected and labeled cells were placed in transwell filter chambers in 0.5% FBS. The inserts were then placed in 24-well plate in 0.5% or 5% FBS. Fluorescence measurements to assess migration of DiI-labeled cells through the transwell membrane were taken. The mean fluorescence of Adβ-gal–infected cells in 0.5% FBS was set at 1 (mean of 3 experiments performed in triplicates). **P < .01 and *P < .05.