Cytotoxic effect of acadesine on B-CLL cells.

(A) Dose-response of acadesine on B-CLL cells. Cells from 5 patients were incubated for 24 hours with various doses of acadesine as indicated. (B) Cells from 70 patients were incubated for 24 hours with or without 0.5 mM acadesine. Viability was measured by analysis of phosphatidylserine exposure and PI uptake as described in “Patients, materials, and methods” and it is expressed as the percentage of nonapoptotic cells. The figure shows results from 20 representative patients.