NBTI, 5-iodotubercidin, and adenosine protect from acadesine-induced apoptosis and AMPK phosphorylation.

(A) B-CLL cells were incubated for 24 hours with 0.5 mM acadesine alone (−) or combined with 10 nM NBTI (N), 0.2 μM 5-iodotubercidin (I), or 0.5 mM adenosine (Ado). Viability was measured as described in “Patients, materials, and methods” and expressed as the percentage of the viability of nontreated cells. Data are shown as the mean value ± SD of 10 patients. (B) B-CLL cells were incubated with 0.5 mM acadesine (indicated as “A”) in the presence or absence of 10 nM NBTI, 0.2 μM 5-iodotubercidine, and 0.5 mM adenosine for 3 hours, and whole-cell extracts were analyzed by Western blot with an antibody against phospho-AMPK (Thr172).