Acadesine does not induce p53 accumulation or phosphorylation.

Cells from B-CLL patients were untreated (C), or treated with 0.5 mM acadesine (A) or 0.8 μM doxorubicin (D) for 24 hours. Cells were lysed and analyzed by Western blot as described in “Patients, materials, and methods.” Total levels of p53 and p53 phosphorylated at Ser15 were analyzed. Results of 3 representative patients of 5 analyzed are shown.