Solid-phase TR6-Fc promotes proliferation of anti-CD3–stimulated total spleen cells and CD4 and CD8 T cells.
Mouse spleen cells (A), CD4 cells (B,C) or CD8 cells (D,E) were stimulated with solid-phase anti-CD3 and TR6-Fc. When anti-CD3 was used at different doses, TR6-Fc and its control, TR11-Fc, were tested at an optimal dose of 10 μg/L. When TR6-Fc was used at different doses, anti-CD3 was used at a suboptimal dose of 0.2 μg/mL. These concentrations referred to those used during the well-coating process. Cell proliferation was measured by 3H-thymidine uptake between 48 and 64 hours after the initiation of culture. Means ± SD of the counts per minute from triplicate samples are shown. The experiments were performed more than 3 times, and a representative set of data is presented.
