AML cell lines show cholesterol increments after therapeutic agent treatments.
AML cell lines (NB4, HL60, KG1a) were treated with equitoxic doses of chemotherapy or radiation, with cholesterol levels subsequently determined by the Amplex Red assay. NB4 and HL60 cells showed significantly higher cholesterol levels 24 hours after γ-irradiation (RAD; 390 cGy) or after 24 hours of treatments with daunorubicin (DNR; 0.05 μM for NB4, 0.01 μM for HL60) or cytarabine (ARA-C; 0.5 μM for NB4, 0.25 μM for HL60). KG1a cells yielded marginally significant cholesterol increments 24 hours after RAD (390 cGy) but not after DNR (0.05 μM) or ARA-C (0.075 μM). Lysates from 105 NB4, HL60, or KG1a cells were used to perform Amplex Red cholesterol assays (100 μL assay volumes). Cellular cholesterol measures are expressed relative to that of untreated cells (UN) from the same lineage. Data are presented with standard errors and represent the mean of 3 to 6 independent replicates.
