TSP-2 fragments do not contain VWF-reducing activity.

(A) VWF (8 nM) was incubated with 0.8, 8, or 80 nM TSP-1, delNo-2, or E3CaG-2. Aliquots of the reactions were analyzed for the average VWF multimer size (■) and for the generation of new thiols in VWF (▪). The dotted lines represent no change in VWF multimer size (top line) or thiols in VWF (bottom line). The asterisks indicate significant difference compared to control (P < .05). (B) Citrated normal plasma (NP) was incubated with 40 nM TSP-1 (lane 2), 400 nM E3CaG-1 (lane 3), or 400 nM E3CaG-2 (lane 4), and aliquots of the reactions were resolved on 1% agarose gel electrophoresis. The VWF was analyzed by Western blot using peroxidase conjugated anti-VWF polyclonal antibodies.