Fig. 2.
Fig. 2. DN T cells infiltrate donor-specific skin allografts and form the dominant subset of graft-infiltrating T cells. / (A) A group of (2C × dm2)F1 mice were given DLI followed by transplantation of both (B6 × Balb/c)F1allogeneic and (B6 × dm2)F1 syngeneic skin grafts. At 21 days (top and middle panels) and 120 days (bottom panel) after transplantation, the accepted skin grafts were harvested and stained with hematoxylin and eosin. In each instance, an overview picture of the skin-graft site is shown in the left panel (× 50) and a close-up view of the deep dermis is shown in the right (× 400). The top panel shows a syngeneic graft at 21 days after transplantation. The epidermis and dermis are normal, and dermal appendages are present (left). In the deep dermis, there is no cellular infiltrate and blood vessels and collagen fibers are normal. The middle panel shows an allograft at 21 days after transplantation. The epidermis shows acanthosis, hyperkeratosis, and focal keratotic plugging. The dermis is edematous and there is a heavy cellular infiltrate in the middle and deep dermis (left). The infiltrate in the deep dermis is predominantly lymphocytic (right). The bottom panel shows an allograft at 120 days after transplantation. The skin structure is normal, except in the deep dermis, where there is a mild cellular infiltrate and fibrosis (left). The deep dermis shows an increase in collagen fibers and is less vascular than normal skin. The infiltrate cells are much less numerous than what was observed at 21 days and are predominantly lymphocytes. (B) Graft-infiltrating cells were collected from the accepted (B6 × BALB/c)F1 skin grafts 7, 14, 58, and 110 days after transplantation. The cells were analyzed by flow cytometry for CD11b+, NK1.1+, γδ-TCR+, CD8+, CD4+, and 1B2+DN T cells. Shown are representative proportions of positive graft-infiltrating cells from at least 3 mice. NT indicates not tested. (C) Graft-infiltrating cells were collected from the DLI-treated (B6 × BALB/c)F1 skin grafts of (2C × dm2)F1 mice 1 week after transplantation. The cells were analyzed by flow cytometry for expression of 1B2, CD4, and CD8. The histogram is gated on the 1B2+ T-cell population.

DN T cells infiltrate donor-specific skin allografts and form the dominant subset of graft-infiltrating T cells.

(A) A group of (2C × dm2)F1 mice were given DLI followed by transplantation of both (B6 × Balb/c)F1allogeneic and (B6 × dm2)F1 syngeneic skin grafts. At 21 days (top and middle panels) and 120 days (bottom panel) after transplantation, the accepted skin grafts were harvested and stained with hematoxylin and eosin. In each instance, an overview picture of the skin-graft site is shown in the left panel (× 50) and a close-up view of the deep dermis is shown in the right (× 400). The top panel shows a syngeneic graft at 21 days after transplantation. The epidermis and dermis are normal, and dermal appendages are present (left). In the deep dermis, there is no cellular infiltrate and blood vessels and collagen fibers are normal. The middle panel shows an allograft at 21 days after transplantation. The epidermis shows acanthosis, hyperkeratosis, and focal keratotic plugging. The dermis is edematous and there is a heavy cellular infiltrate in the middle and deep dermis (left). The infiltrate in the deep dermis is predominantly lymphocytic (right). The bottom panel shows an allograft at 120 days after transplantation. The skin structure is normal, except in the deep dermis, where there is a mild cellular infiltrate and fibrosis (left). The deep dermis shows an increase in collagen fibers and is less vascular than normal skin. The infiltrate cells are much less numerous than what was observed at 21 days and are predominantly lymphocytes. (B) Graft-infiltrating cells were collected from the accepted (B6 × BALB/c)F1 skin grafts 7, 14, 58, and 110 days after transplantation. The cells were analyzed by flow cytometry for CD11b+, NK1.1+, γδ-TCR+, CD8+, CD4+, and 1B2+DN T cells. Shown are representative proportions of positive graft-infiltrating cells from at least 3 mice. NT indicates not tested. (C) Graft-infiltrating cells were collected from the DLI-treated (B6 × BALB/c)F1 skin grafts of (2C × dm2)F1 mice 1 week after transplantation. The cells were analyzed by flow cytometry for expression of 1B2, CD4, and CD8. The histogram is gated on the 1B2+ T-cell population.

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