Susceptibility of human cell lines to CD47-induced cell death: lack of correlation between CD47 expression and CD47-induced cell death.

Various cell lines were stained for CD47 expression using B6H12 mAb: isotype-control mAb (open histogram) and CD47 mAb (shaded histogram). Cells were cultured overnight on immobilized control or CD47 mAb analyzed by flow cytometry. Shown are the mean percents of CD47-induced cell death, calculated as indicated in “Patients, materials, and methods.”