Fig. 5.
![Fig. 5. Down-regulation of CD47-induced PS exposure by K+ efflux or antimycin A treatment: lack of effect of PKC inhibitor rottlerin. / Freshly isolated B-CLL cells and the U937 cell line were cultured with or without immobilized CD47 mAbs. (A) Cultures were performed in RPMI medium containing normal [Na+] or inverted [Na+]/[K+] ratios [K+]. Shown are percent of viable cells (annexin-V−/PI−). (B-C) Cells were cultured in the presence or absence of immobilized CD47 mAbs with or without antimycin A (30 μM) or increasing concentrations of rottlerin. Shown is the percent of dead cells (annexin-V+ or DiOC6low cells); one representative experiment of 4 is shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/100/8/10.1182_blood-2001-12-0217/4/h82023277005.jpeg?Expires=1735511272&Signature=MHd8M-8vk2bpfwjx9-CcvwXcELa3V2IqZl7VeMohEcNVl2H7GzMRoK2sGAf~NNy4gduO2RkOizXj3CDE15A6-~uxW9GAzoCHm~OIjmm6PWjJSDUcPDbiOleQnya8PPFBE41CFGPYgAS3~Vwi57hd53Lbaa7bTYHsyusbbUx4RLAjgECgN3fIsTPX117oQBgP09akhSxGmI2z0aPSq7XYEUZrZCdcBrzVLpm~pjOsKAgpUVPcSccXJBcCyIcZnVuwoDQWS4wjf2kpUbruLOpQpNKPiLlQpzGUto-rz2xKzPsmvO7pvA-0tDIA1fAa3R~~92Um~4ZzxRSPUVELSwqPbQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Down-regulation of CD47-induced PS exposure by K+ efflux or antimycin A treatment: lack of effect of PKC inhibitor rottlerin.
Freshly isolated B-CLL cells and the U937 cell line were cultured with or without immobilized CD47 mAbs. (A) Cultures were performed in RPMI medium containing normal [Na+] or inverted [Na+]/[K+] ratios [K+]. Shown are percent of viable cells (annexin-V−/PI−). (B-C) Cells were cultured in the presence or absence of immobilized CD47 mAbs with or without antimycin A (30 μM) or increasing concentrations of rottlerin. Shown is the percent of dead cells (annexin-V+ or DiOC cells); one representative experiment of 4 is shown.
