Fig. 2.
Effect of LY294002 and rapamycin on phosphorylation.
LY294002 inhibits phosphorylation of Akt, P70S6K, and FKHRL1 proteins in human erythroid progenitors and UT7 cells whereas rapamycin inhibits only the mTOR/P70S6K pathway. Lysates were prepared from human erythroid progenitors or UT7 cells stimulated with Epo in the absence or presence of either LY294002 or rapamycin at different concentrations, and analyzed by Western blot with anti-pAkt (Ser473), anti-pP70S6K (Thr389), anti-pFKHRL1 (Thr32), anti-Akt, anti-P70S6K, and anti-FKHRL1 antibodies.
