Fig. 4.
Phosphorylation of Gab2 protein requires either tyrosine 343 or tyrosine 401 of the EpoR.
Ba/F3 cells expressing either wild-type EpoR or mutants of the EpoR (Tyr1, Tyr1-Phe2-Tyr3-8, Phe1-Tyr2-8, Zero, Phe1-Phe2-Tyr3-8) were stimulated with Epo at 10 U/mL for 10 minutes. Lysates from 15 × 106 cells were immunoprecipitated with anti-Gab2 antibodies and analyzed by Western blot with the use of antiphosphotyrosine antibodies (anti-PY).
