Fig. 8.
![Fig. 8. Effect of LY294002 on proliferation of human erythroid progenitors and UT7 cells. / Inhibition of PI 3-K/Akt pathway by LY294002 dramatically inhibits proliferation of both human erythroid progenitors and UT7 cells. D7 + 1 human erythroid progenitors and UT7 cells were cultured at 105/mL in serum-free medium with Epo at 2 U/mL, without or with LY294002 (at 50 μM) or rapamycin (at 50 ng/mL) for 48 hours. DNA synthesis was assessed by [3H] thymidine incorporation after 4 hours. Analysis is given as the mean of 3 separate experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/9/10.1182_blood-2002-07-2332/4/h80934214008.jpeg?Expires=1735559713&Signature=qERg18bfvBHueACETIY912AeDnAV5DIoN5WyR~wi2ySPG0Kk7DUYOkexpYv~srW08b9Y02fv-YBgu1H2rE0Upp3AIOwB9ZLZPfY~EjdxlhHU-YSc2kN0HRGvN9oVsbmOlhHAO7ZmtlXrt1oD19McgL4nq8CT6xAmR3sFWdOJOA4jEjSftSVE9PA5Df-FT86Y1NpPdrscQ~rEgk0LQAY~OPplqT8LYIb1OvKFcfi9fXKPJQ2TVhpZcpCyrZ~ho5e8KEPo3xXp4syVbxvnevJSqRYa4PHLZGu7-7UFgSpOhPZxQv1UHHFrD7q4lNbpCOHWNJRyBocicMP5O4DkYXMuiw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of LY294002 on proliferation of human erythroid progenitors and UT7 cells.
Inhibition of PI 3-K/Akt pathway by LY294002 dramatically inhibits proliferation of both human erythroid progenitors and UT7 cells. D7 + 1 human erythroid progenitors and UT7 cells were cultured at 105/mL in serum-free medium with Epo at 2 U/mL, without or with LY294002 (at 50 μM) or rapamycin (at 50 ng/mL) for 48 hours. DNA synthesis was assessed by [3H] thymidine incorporation after 4 hours. Analysis is given as the mean of 3 separate experiments.
