NK92 cells transduced with p210 become IL-2 independent.

Untransduced (parental) NK92 cells were transduced with a control vector (eGFP) and a vector containing BCR/ABL (p210). Parental and sorted eGFP⁺ (termed eGFP) and p210-eGFP⁺ NK92 cells (termed p210⁺) (3 × 10⁵ cells/mL) were cultured in the presence (▨) or absence (▪) of 500 U/mL IL-2, and the number of viable cells was determined after 14 days of culture. Parental and eGFP⁺control NK92 cells died in the absence of IL-2, whereas p210⁺ cells without IL-2 expanded at similar rates as parental NK92 cells in the presence of IL-2.