Natural cytotoxicity of p210⁺ NK92 cells is decreased.

Untransduced (parental), eGFP⁺, and p210-eGFP⁺(p210⁺) NK92 cells were tested against the K562 targets, at effector-to-target ratios ranging from 20:1 to 0.08:1. eGFP⁺ and p210⁺ cells were cultured in the presence or absence of 500 U/mL IL-2 between 14 days and up to 12 months before cytotoxicity testing, and results at all time points were similar. The difference between p210⁺cells (with or without IL-2) and eGFP⁺ cells with IL-2 is highly significant at all but the lowest E/T ratio (P < .01). Data shown as the mean ± SEM of 5 different experiments performed in triplicate.