KIR expressing p210⁺ NK92 cells derive from the same clone.

In a Southern blot analysis, genomic DNA from parental, eGFP⁺, and p210-eGFP⁺(p210⁺) NK92 cells was digested withEcoRI, electrophoresed on a 0.7% agarose gel, and probed with a ³²P-eGFP probe. Representative lanes show that CD158b/j⁺/CD158e⁻ (lane 4), CD158b/j⁺/CD158e⁺ (lane 5), and CD158b/j⁻/CD158e⁻ (lane 6) p210⁺NK92 cell populations express identical dominant bands, indicating that they derive from the same clone. KIR-negative eGFP⁺ NK92 cells show a smear (lane 1), and 2 different p210⁺ clones show a different unique band (lanes 2-3).