Adhesion and transmigration capacities on Fn of synchronized CD34+ cells.
Cells were prestimulated with SCF, FL, and TPO during 16 hours, after which they were treated for 24 hours with 2 μg/mL aphidicolin. Cells were then washed and replated in fresh medium and cytokines to allow cell cycle progression. Cells were sampled after 2, 4, 6, 8, and 24 hours following aphidicolin treatment (n = 3). (A) Binding to Fn and BSA was determined at each time point. *P < .05 compared with uncultured cells (−40 hours); #P < .05 compared with cells blocked by aphidicolin in G1/S (0 hours). (B) Transmigration assays were carried out across Fn toward MS-5 CM (gray bars) and control medium (white bars) or across BSA toward MS-5 CM (black bars). *P < .05 compared with uncultured cells.
