Liquid suspension culture of CD34⁺KDR⁺ cells generating hematopoietic and endothelial progeny.

(A) Number of cells in FCS⁻ liquid suspension culture of CB CD34⁺KDR⁺ (○,■) vs CD34⁺KDR⁻ (▵) cells supplemented with VEGF. Starting from day 14, the number of small blastlike (○) and larger (■) cells in KDR⁺ culture is indicated. A representative experiment is shown. (B) Immunocytochemistry analysis of cells in CD34⁺KDR⁺ culture at day 14 by combined staining with CD45 (red) and VWF (brown). Right panel shows a double-stained cell (original magnification, × 1000).