Growth of hemangioblast siblings in different culture conditions.

(A) Single CB CD34⁺KDR⁺ cells generated 4-cell clones. Sibling cells from each clone were seeded in Hem, Hem-End, or End medium (left). In 2 representative experiments (a, clones 1-71; b, clones 1-93) we show the total number of Hem and Hem-End colonies and specify the clones (23a, 35a; 15b, 49b) generating Hem-End or Hem colonies in Hem-End or Hem medium, respectively. One of these clones (23a) generated a small Hem-End cell cluster in End medium. (B,C) Immunofluorescence and RT-PCR analysis of Hem-End colonies generated by a and b clones.