Fig. 5.
![Fig. 5. Distinct inhibition of [Ca++]ielevations in platelets interacting with immobilized VWF under flow. / A blood cell suspension prepared as described in the legend for Figure2 was perfused for 90 seconds over immobilized VWF at a shear rate of 3000 seconds−1. The diagrams show the [Ca++]i changes in all surface-interacting platelets during translocation or stationary adhesion for 30 seconds. Noteworthy are the presence of α/β peaks and γ peaks (arrows) in the control experiment, the selective absence of γ peaks in the presence of wortmannin (100 nM) or apyrase (10 U/mL), and the absence of any Ca++ elevation in the presence of sodium nitroprusside (5 μM). Similar results were obtained in 4 different experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/100/8/10.1182_blood-2002-02-0514/4/h82023252005.jpeg?Expires=1735537942&Signature=akLIZVnVot6q1oCT89ulHjC3iKaffcmjJdwd7EWyMBt2lpb1r84UP5~d6PKLh5MjkYrJZvHcoP8GANdZC7am9LL-fM3vr0DFIv39yr0kgFHC7SdB8bIhIz26P1E3u9P4A9dS4FsG0Z3I-dzNSzPsaiFOy~RzvFiIKkSGoxYzKTTI1d84Jb6InGyuj~PQ9A6AMIBVe0FQ5VDL8~qb9tmNSYcqa5zI72gjsu6LEGpk8nlQA5osni21lXzLqjx9nsbre-a~sAQf5fI27DUbHVbqccMN7udWco46-dTXhcAX0ZuaY8e2yfSa9eH3A7mYHqpDLA91ie~nvULTWVv08mrRCA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Distinct inhibition of [Ca++]ielevations in platelets interacting with immobilized VWF under flow.
A blood cell suspension prepared as described in the legend for Figure2 was perfused for 90 seconds over immobilized VWF at a shear rate of 3000 seconds−1. The diagrams show the [Ca++]i changes in all surface-interacting platelets during translocation or stationary adhesion for 30 seconds. Noteworthy are the presence of α/β peaks and γ peaks (arrows) in the control experiment, the selective absence of γ peaks in the presence of wortmannin (100 nM) or apyrase (10 U/mL), and the absence of any Ca++ elevation in the presence of sodium nitroprusside (5 μM). Similar results were obtained in 4 different experiments.
