Fig. 3.
Characterization of CD4+CD25+ T cells generated from the primed human naive CD4+ T cells with allogeneic regulatory DCs.
(A-B) Human allogeneic naive CD4+ T cells (5 × 106) were cultured with allogeneic normal or regulatory DCs (5 × 105) for 5 days, and CD4+ T cells isolated from the coculture were assayed for phenotype (A) and cytokine profile (B) by FACS. The results are representative of 5 experiments with similar results, and data are represented by a dot plot. (C) Human Ag-primed CD4+ T cells were isolated from the coculture of human naive CD4+T cells (5 × 106) with allogeneic normal mDCs (5 × 105) for 3 days. CD4+CD25+T cells were isolated from the coculture of human naive CD4+ T cells (5 × 106) with allogeneic regulatory iDCs (5 × 105) for 5 days. Human Ag-primed CD4+ T cells or CD4+CD25+ T cells (105) were cultured with allogeneic normal mDCs (104) in the presence or absence of different numbers of CD4+CD25+ T cells or Ag-primed CD4+T cells, and the proliferative response was measured on day 5. Data were expressed as mean values ± SD of 5 individual experiments. *P < .01 compared with Ag-primed CD4+ T cells plus allogeneic normal mDCs by Student paired ttest. Error bars express SD of mean values.