American Society of Hematology

Fig. 1.

$Fig. 1. Human uPA gene regulatory region and Northern blot analysis of the steady-state uPA mRNA level in HeLa and PC3 cells. / (A) Scheme of the uPA regulatory region, depicting enhancer (E, ■) and proximal promoter (PP, ▪). The first enlargement shows the schematic position of the low- and high-affinity Sp1 sites and of the TATA box in the PP region. The second enlargement shows the actual sequence of the −86/+32 region, where the Sp1 sites are indicated in bold and the TATA box in italics. The sequence comprised between −80 and −30 represents the oligonucleotide used for mobility shift assays (see below). (B) Total mRNA of 30 μg was fractionated on a 1% (wt/vol) agarose/formaldehyde gel. Following transfer to a positively charged nylon membrane, the Northern blot analysis was sequentially probed with uPA cDNA and GAPDH probes. uPA mRNA is detectable only in PC3 cells, which constitutively express the uPA gene.$

Human uPA gene regulatory region and Northern blot analysis of the steady-state uPA mRNA level in HeLa and PC3 cells.

(A) Scheme of the uPA regulatory region, depicting enhancer (E, ■) and proximal promoter (PP, ▪). The first enlargement shows the schematic position of the low- and high-affinity Sp1 sites and of the TATA box in the PP region. The second enlargement shows the actual sequence of the −86/+32 region, where the Sp1 sites are indicated in bold and the TATA box in italics. The sequence comprised between −80 and −30 represents the oligonucleotide used for mobility shift assays (see below). (B) Total mRNA of 30 μg was fractionated on a 1% (wt/vol) agarose/formaldehyde gel. Following transfer to a positively charged nylon membrane, the Northern blot analysis was sequentially probed with uPA cDNA and GAPDH probes. uPA mRNA is detectable only in PC3 cells, which constitutively express the uPA gene.

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