American Society of Hematology

Fig. 5.

$Fig. 5. Sp1 is phosphorylated in PC3 but not HeLa cells. / Nuclear extracts were treated with alkaline phosphatase for 45 minutes at 37°C prior to fractionation on an 8% SDS-polyacrylamide gel for 3 hours at 150 V. Transfer of proteins to PVDF membrane and overlay with anti-Sp1 polyclonal antibodies was as described in Figure 4. The upper band appearing in the PC3 nuclear extracts (P-Sp1), which disappears after alkaline phosphatase treatment, indicates that Sp1 is phosphorylated in this cell line.$

Sp1 is phosphorylated in PC3 but not HeLa cells.

Nuclear extracts were treated with alkaline phosphatase for 45 minutes at 37°C prior to fractionation on an 8% SDS-polyacrylamide gel for 3 hours at 150 V. Transfer of proteins to PVDF membrane and overlay with anti-Sp1 polyclonal antibodies was as described in Figure 4. The upper band appearing in the PC3 nuclear extracts (P-Sp1), which disappears after alkaline phosphatase treatment, indicates that Sp1 is phosphorylated in this cell line.

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