Effect of anti-Ly49G2 (mAb 4D11) treatment on Ly49C/I+, Ly49G2+, and Ly49C/I+xLy49G2+ NK cell numbers in the spleens of C57BL/6 (H2b) mice.
Mice were treated as described in “Materials and methods.” Flow cytometric analysis of fresh spleen cells of control mice, mice treated with mAb 5E6 (anti-Ly49C/I) or mAb 4D11 (anti-Ly49G2), stained with goat–anti-mouse or goat–anti-rat IgG, anti-Ly49C/I (mAb 5E6), anti-Ly49G2 (mAb 4D11), or anti-Ly49D (mAb 4E5), anti-CD3 and pan NK mAb NK1.1. Panel A represents staining flow profile of NK1.1+ CD3− gated spleen cells stained with goat–anti-mouse IgG (gray line) for in vivo mAb 5E6–treated mice and with goat–anti-rat IgG (gray line) for in vivo mAb 4D11–treated mice. The black lines in panel A represent staining of NK1.1+ CD3− gated spleen cells of control mice with goat–anti-mouse IgG or goat–anti-rat IgG. Panel B represents staining flow profile from a single mouse and panel C represents average percentages (mean ± SD, 5 mice per group) of NK1.1+ gated Ly49C/I+(5E6+), Ly49G2+(4D11+), Ly49D+(4E5+), and Ly49C/I+(5E6+) × Ly49G2+(4D11+) or Ly49D+(4E5+) × Ly49G2+(4D11+) NK cell population of the same experiment. The experiment was done 2 times.
