Effect of anti-Ly49G2 (mAb 4D11) or anti-Ly49C/I (mAb 5E6) treatment and irradiation on NK percentage and cell numbers in the spleens of C57BL/6 (H2b) mice.
Groups of 10 C57BL/6 (H2b) mice were injected intraperitoneally with 500 μg mAb 5E6 (anti-Ly49C/I) or 200 μg mAb 4D11 (anti-Ly49G2) or 0.2 mL NRS. Forty-eight hours later, 5 mice from each group were lethally irradiated at 950 cGy and 24 hours after irradiation (total 72 hours since antibody treatments) spleens from all the mice were harvested, each group of spleens pooled, and single-cell suspension prepared. Four-color flow cytometric analysis was performed as mentioned in “Materials and methods.” The figure represents NK percentage (A) and total NK cell numbers (B) in the spleens of nonirradiated and irradiated mice.
