Effect of anti-Ly49G2 (mAb 4D11) or anti-Ly49C/I (mAb 5E6) treatment and irradiation on Ly49C/I⁺, Ly49G2⁺ NK cell numbers in the spleens of C57BL/6 (H2^b) mice.

Groups of 10 C57BL/6 (H2^b) mice were injected intraperitoneally with 500 μg mAb 5E6 (anti-Ly49C/I) or 200 μg mAb 4D11 (anti-Ly49G2) or 0.2 mL NRS. Forty-eight hours later, 5 mice from each group were irradiated at 950 cGy and 24 hours after irradiation spleens from all the mice were harvested (total 72 hours since antibody treatments), each group of spleens pooled and single-cell suspension was prepared. Four-color flow cytometric analysis was performed as mentioned in “Materials and methods.” Panels A and B represent NK1.1⁺ CD3⁻-gated Ly49C/I⁺(5E6⁺) and Ly49G2⁺(4D11⁺) NK cell populations in the spleens of nonirradiated and irradiated mice.