NF-AT reporter activity but not AP-1 is inhibited by RosA.

(A-B) Jurkat cells (2 × 10⁶) were transfected with NF-AT (A) or AP-1 (B) reporter. Twenty-four hours after transfection, Jurkat cells were preincubated in the presence or absence of RosA (30 μM) for 2 hours and then stimulated with immobilized anti-CD3 mAb (UCHT1, 5 μg/mL) (░) or PMA (5 ng/mL) and ionomycin (0.5 μg/mL) (▨) for 16 hours. In the RosA-treated group, RosA was present throughout the whole 16 hours of incubation. Jurkat cells without any stimulation are marked as open bars (■). Shown are the relative light units (RLU), indicated as the average RLU and standard error of 2 independent experiments.