Fig. 7.
RosA does not suppress the TCR-induced tyrosine/threonine phosphorylation of Erk.
Jurkat cells were preincubated with the indicated concentrations of RosA for 4 hours and kept unstimulated or stimulated for 2 minutes as indicated in “Materials and methods.” Cells approximately of 2 × 106 were lysed in lysis buffer and resolved by 12% SDS-PAGE. The blot was probed with antiphospho-Erk1/2 mAb or rabbit polyclonal anti-ERK1/2 antibody. Erk1/2 of p44 and p42 are indicated by arrows. Results are representative of 2 separate experiments.
