Effects of activation of hGM (M) Rα, hβcand hGM/βc, hβc on cell development.
(A) Cell morphology. (i) hGM (M) Rα, hβc and (ii) hGM/βc, hβc cells were cultured in hGM-CSF (1 ng/mL) for 60 days and cytospin samples prepared at intervals during this time. Morphology was assessed following May-Grünwald-Giemsa staining. The morphology results are expressed as percentage of the total cells scored and pooled data from 3 experiments. The SEMs were less than 10%. (B) Ability to differentiate following long-term culture in hGM-CSF. Photomicrographs are shown of (i) hGM (M) Rα, hβc cells and (ii) hGM/βc, hβc cells cultured in hGM-CSF (1 ng/mL) for 60 days. After this time, (iii) hGM (M) Rα, hβc cells and (iv) hGM/βc, hβc cells were harvested, washed, and cultured in murine cytokines that promote myeloid development for a further 7 days. Cytospin preparations were May-Grünwald-Giemsa stained and are representative of 3 separate experiments. Bar is 10 μm.
