Messenger RNA expression of alternative transcripts of CD79, ΔCD79a and ΔCD79b.

Shown are DNA fragments resulting from semiquantitative RT-PCR of RNA isolated from malignant B cells with primers specific for CD79a, CD79b, or glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Table 1). Messenger RNA was first isolated from samples and then reverse transcribed to yield cDNA for PCR. Primers for GAPDH were used to verify integrity and quantity of cDNA. (A) RT-PCR from 16 B-CLL samples. (B) RT-PCR from a selection of other B-cell malignancies: follicle center cell lymphoma (FCL) (lanes 1-3); diffuse large B-cell lymphoma (DLCL) (lanes 4-11); myeloma (lanes 12-16); splenic lymphoma with villous lymphocytes (SLVL) (lanes 17-19); and hairy cell leukemia (HCL) (lanes 20-22). The ratios in each panel show the relative level of ΔCD79b/CD79b.