Fig. 6.
Sensitivity of Ramos-EHRB clones to anti-CD20 mAb following transfection with ΔCD79b.
Four ΔCD79b-transfected clones of Ramos-EHRB were cultured (5 × 105/mL) in the presence of anti-CD20 mAb for 48 hours before assessing apoptosis via flow cytometry using the annexin V–FITC and PI assay as detailed in Figure 2. Underneath, the lower panel shows the levels of surface CD20 on wild-type and ΔCD79b-transfected Ramos-EHRB cells as measured by flow cytometry. The 4 clones, 4, 9, 12, and 23, were stained with FITC-conjugated mAb to CD20 (AT80), washed, and then assessed by flow cytometry. The solid histogram represents cells stained with an irrelevant control mAb (CP1/17). The 2 least intense FACS profiles represent clones 4 and 12.
