Effect of deficiency of SLP-76 on annexin V binding.

Annexin V binding to SLP-76^+/− (A) or SLP-76^−/− (B) platelets was measured by flow cytometry in unstimulated platelets (dotted line) or after stimulation with thrombin (solid line), or convulxin (dashed line). (C) The percentage of platelets staining positively for annexin V binding, defined as region M1 as shown in panels A and B, was determined for SLP-76^+/− (open bars; n = 7) or SLP-76^−/−(filled bars; n = 7) platelets that were either left unstimulated (control) or stimulated with thrombin (0.5 U/mL), convulxin (250 ng/mL), or ionomycin (3 μM). *P < .05 versus control.