EVI1 mRNA expression in AML samples as determined by Northern blotting.

Human 600-bp HindIII-Ncol EVI1 probe (see Figure 1) was used, which does not discriminate betweenEVI1 and MDS1-EVI1. Murine GAPDH fragment was used as control. Patients 2, 3, and 7 carry a 3q26 abnormality (Table 3); patients 8, 9, and 34 have high EVI1expression but no 3q26 abnormality (Table 4). C1, C2, C3 represent AML patients without EVI1 expression. N1 and N2 represent normal bone marrow samples.