Fig. 2.
Effect of IL-15 and IL-12 stimulation on induction of 4-1BB in CD8+ T cells.
IL-15 and IL-12 stimulation is required for induction of 4-1BB on CB but not on PB CD8+ T cells. Purified CD8+ T cells from CB and PB were cultured for 5 days in the presence or absence of IL-15 and IL-12 followed by a 3-day stimulation with anti-CD3 as described in “Materials and methods.” The resulting cells were analyzed for 4-1BB expression by staining with anti–4-1BB–PE as shown by filled histograms. The open histograms indicate staining with the isotype control mouse IgG1-PE. The percentages shown indicate the 4-1BB+ CD8+T cells.
