RT-PCR analysis of the SPAN-Xb gene in patients with hematologic malignancy using a pair of sequence-specific primers.

SPAN-Xb mRNA could be detected only in patients with hematologic malignancy and not in the peripheral blood or bone marrow of healthy donors. Lane 1, peripheral blood from a healthy donor; lane 2, bone marrow from a healthy donor; lanes 3-6, tumor cells from patients with AML (lane 3), CLL (lane 4), CML (lane 5), and MM (lane 6); lane 7, normal testis RNA; lane 8, positive control amplification using a plasmid containing SPAN-Xb cDNA. M indicates molecular marker; lane a, PCR of DNase I-treated RNA (ie, PCR without RT); lane b, PCR of RNA that underwent RT; and lane c, control amplification for β-actin gene fragment.