American Society of Hematology

Fig. 4.

$Fig. 4. Successful generation of SPAN-Xb recombinant protein fromE coli. / (A) Coomassie blue staining of a 12% SDS-polyacrylamide gel, under reduced conditions, showing purities of different aliquots of recombinant SPAN-Xb protein. (B) Western blot analysis of different aliquots of recombinant SPAN-Xb protein using anti-His tag antibodies, confirming the successful generation of recombinant SPAN-Xb protein. M indicates protein marker; lanes 1-2, recombinant protein from 2 different fractions of the eluates; lane 3, lysate of flow-through from washing; lane 4, lysate after passage through affinity column; lanes 5-6, different aliquots of recombinant E coli lysates; lane 7, lysate after 4 hours of protein induction; and lane 8, lysate at time 0 of protein induction.$

Successful generation of SPAN-Xb recombinant protein fromE coli.

(A) Coomassie blue staining of a 12% SDS-polyacrylamide gel, under reduced conditions, showing purities of different aliquots of recombinant SPAN-Xb protein. (B) Western blot analysis of different aliquots of recombinant SPAN-Xb protein using anti-His tag antibodies, confirming the successful generation of recombinant SPAN-Xb protein. M indicates protein marker; lanes 1-2, recombinant protein from 2 different fractions of the eluates; lane 3, lysate of flow-through from washing; lane 4, lysate after passage through affinity column; lanes 5-6, different aliquots of recombinant E coli lysates; lane 7, lysate after 4 hours of protein induction; and lane 8, lysate at time 0 of protein induction.

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