Fig. 2.
Fig. 2. Generation of 32D/Jak3 cells. / (A) Expression vectors used in the generation of 32D/Jak3 cells. Two independent 32D/Jak3 single-cell clones representing the 2 expression vectors, no. 10.3 (pcDNA3Jak3) and no. 1.1 (pOPI3Jak3) were selected for further analysis. (B) Overexpression of Jak3 in 32D/Jak3 cells. Parental 32Dcl3 and 32D/Jak3 (clone nos. 1.1, 1.2, 10, 10.3) cell lysate was used for immunoprecipitation with either normal rabbit serum (PI) or an anti-Jak3 antibody. The immunoprecipitates were immunoblotted with anti-Jak3 (upper panel) and 4G10 (lower panel) antibodies. (C) Increased cell survival in 32D/Jak3 cells under conditions of IL-3 deprivation. Cells were washed free of IL-3 and plated in medium without IL-3 for 4 days and subjected to morphologic analysis on staining with May-Grünwald-Giemsa staining. Original magnification × 10.

Generation of 32D/Jak3 cells.

(A) Expression vectors used in the generation of 32D/Jak3 cells. Two independent 32D/Jak3 single-cell clones representing the 2 expression vectors, no. 10.3 (pcDNA3Jak3) and no. 1.1 (pOPI3Jak3) were selected for further analysis. (B) Overexpression of Jak3 in 32D/Jak3 cells. Parental 32Dcl3 and 32D/Jak3 (clone nos. 1.1, 1.2, 10, 10.3) cell lysate was used for immunoprecipitation with either normal rabbit serum (PI) or an anti-Jak3 antibody. The immunoprecipitates were immunoblotted with anti-Jak3 (upper panel) and 4G10 (lower panel) antibodies. (C) Increased cell survival in 32D/Jak3 cells under conditions of IL-3 deprivation. Cells were washed free of IL-3 and plated in medium without IL-3 for 4 days and subjected to morphologic analysis on staining with May-Grünwald-Giemsa staining. Original magnification × 10.

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