Fig. 3.
Fig. 3. Acceleration of differentiation in G-CSF–stimulated 32D/Jak3 cells. / (A) 32Dcl3 cells or 32D/Jak3 no. 10.3 cells were washed free of IL-3 and plated in the presence of G-CSF. Aliquots of the cell cultures were cytocentrifuged at the indicated days after treatment with G-CSF. The progression of granulocytic differentiation was monitored by May-Grünwald and Giemsa staining. Original magnification × 20. (B) Aliquots of the cell cultures were cytocentrifuged at the indicated days after treatment with G-CSF. The distribution of cells at different stages of granulocyte differentiation was determined by counting at least 100 cells in a field. (C) Cessation of proliferation followed by acceleration of granulocytic differentiation of 32D/Jak3 cells in response to G-CSF. 32Dcl3 cells or 32D/Jak3 no. 10.3 cells were washed free of IL-3 and plated in the presence of G-CSF. Cell viability was monitored using trypan blue staining.

Acceleration of differentiation in G-CSF–stimulated 32D/Jak3 cells.

(A) 32Dcl3 cells or 32D/Jak3 no. 10.3 cells were washed free of IL-3 and plated in the presence of G-CSF. Aliquots of the cell cultures were cytocentrifuged at the indicated days after treatment with G-CSF. The progression of granulocytic differentiation was monitored by May-Grünwald and Giemsa staining. Original magnification × 20. (B) Aliquots of the cell cultures were cytocentrifuged at the indicated days after treatment with G-CSF. The distribution of cells at different stages of granulocyte differentiation was determined by counting at least 100 cells in a field. (C) Cessation of proliferation followed by acceleration of granulocytic differentiation of 32D/Jak3 cells in response to G-CSF. 32Dcl3 cells or 32D/Jak3 no. 10.3 cells were washed free of IL-3 and plated in the presence of G-CSF. Cell viability was monitored using trypan blue staining.

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