Fig. 7.
Fig. 7. Acceleration of morphologic differentiation in GM-CSF–stimulated BM-Jak3 cells. / Myeloblast-enriched BM cells from wild-type (C57/BL6) mice were infected with the retroviral vectors pMSCV-Jak3/neo or pMSCV-neo. Cells were seeded in methylcellulose and selected in the presence of G418. Several colonies from each infection were expanded, transferred to liquid culture, and induced for differentiation with GM-CSF. Cells were cytocentrifuged at the indicated time points and progression of differentiation was monitored by May-Grünwald and Giemsa staining. Original magnification × 20.

Acceleration of morphologic differentiation in GM-CSF–stimulated BM-Jak3 cells.

Myeloblast-enriched BM cells from wild-type (C57/BL6) mice were infected with the retroviral vectors pMSCV-Jak3/neo or pMSCV-neo. Cells were seeded in methylcellulose and selected in the presence of G418. Several colonies from each infection were expanded, transferred to liquid culture, and induced for differentiation with GM-CSF. Cells were cytocentrifuged at the indicated time points and progression of differentiation was monitored by May-Grünwald and Giemsa staining. Original magnification × 20.

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