TNF-α stimulation followed by anti–Ly-6G antibody induces microvascular arrest and coagulation in the mouse cremaster muscle.
(A) Change in venular center-line blood flow velocity induced by RB6-8C5 mAb in unstimulated (⋄) and TNF-α–stimulated (▴) mice and by control IgG (░) in TNF-α–stimulated mice. Data are shown as means ± SEMs of 6 venules from 6 mice. (B) Change in venular center-line blood flow velocity induced by mAb 1A8 (○) against Ly-6G and by Fab (■) and F(ab′)2 (♦) fragments of antibody RB6-8C5. Data shown are representative of 3 or 4 mice per treatment. (C) Effect of RB6-8C5 in mice treated for 4 hours with 100 ng (●) or 20 ng (⋄) TNF-α. Data shown are representative of 3 or 4 mice per treatment. (D) Effect of RB6-8C5 in C3H/HeJOlaHsd-LPSd(LPS-resistant) mice stimulated for 4 hours with TNF-α (500 ng, intrascrotal injection). Data are shown as means ± SEMs of 6 venules from 6 mice. (E) Appearance of a typical venule 4 hours after TNF-α. The same vessel 2 to 3 minutes after RB6-8C5 (F) and 5 minutes after RB6-8C5 (G). The scale bar below panel E measures 50 μm and applies to all three images. A time-lapse movie (Video 1) of panels E to G is included with the online version of the manuscript.
