Measurements of affinities between GST-SH2-SHIP fusion protein and phosphopeptides by surface plasmon resonance.

Biotinylated phosphopeptides indicated were captured on a streptavidin-coated sensor chip, and GST-SH2-SHIP was injected for 5 minutes at a flow rate of 30 μL/min at 25°C. The chip was washed with binding buffer for a further 5 minutes to examine dissociation rates.