The introduction of SH2-SHIP enhances the phagocytic abilities in the absence of FcγRII(b).

(A) THP-1 cells were transiently transfected with GFP or GFP-SH2-SHIP. The GFP-positive cells were sorted and analyzed by FACS analysis. Dashed lines indicate untransfected THP-1 cells. (B) The sorted GFP-positive cells were incubated with RBCs coated with Fab fragments of IV.3 antibody for 20 minutes at 37°C. The results were expressed as the number of the internalized RBCs per 100 cells which phagocytosed at least one RBC (phagocytic index). Open and closed bars represent phagocytic indexes for GFP-expressing cells and GFP-SH2-SHIP–expressing cells, respectively. Results are shown as the averages of duplication and are representative of 2 independent experiments. Bars represent standard errors of duplicate samples.