Adhesion of bone marrow stem and progenitors cells to laminin isoforms and fibronectin.

Adhesion of human bone marrow CD34⁺ cells (A,D), CD34⁺CD38⁻ cells (B), and CD34⁺CD38⁺ cells (C) to fibronectin (FN), laminin-1 (LN-1), laminin-8 (LN-8), and laminin-10/11 (LN-10/11). PBS indicates cell adhesion to wells coated with PBS instead of proteins; light columns, cell adhesion without treatment with TPA (n = 9); dark columns, cell adhesion after 1 hour of treatment with TPA (PBS, n = 6; other columns, n = 9; values are shown as means ± SDs); asterisks, a significant difference from the corresponding control value (percentage of adherent cells in wells coated with PBS); ns, not significant; *P < .05; **P < .01; ***P < .001. (D) CD34⁺ cell adhesion to wells coated with 10 μg/mL and 30 μg/mL proteins. The difference in cell adhesion to each protein at 10 μg/mL and 30μg/mL was not significant (P > .05).