Inhibition of adhesion of bone marrow stem and progenitor cells to laminin-10/11 and laminin-8 by monoclonal antibodies against integrin α6 and β1 chains.

Adhesion of CD34⁺ cells (A,B) and CD34⁺CD38⁻ cells (C-E) to laminin-10/11 (LN-10/11; A,C,D) and laminin-8 (LN-8; B,E). PBS indicates cell adhesion to wells coated with PBS instead of proteins. Adhesion assay was performed in the presence of antibodies GoH3 against integrin α6 and P4C10 against β1 chain (int.α6 and int.β1, respectively), isotype-matched control rat or mouse monoclonal antibodies (rIgG and mIgG, respectively), or 10 mmol EDTA. No ab indicates cell adhesion to laminin-10/11 or laminin-8 without antibody addition. The results are shown as percentages of adhesion to laminin-10/11 or laminin-8 without antibody addition. There was a significant difference in cell adhesion in the presence of anti-integrin antibodies or EDTA compared with adhesion to laminins without antibody addition (**P < .01; ***P < .001). Shown are means of 1 experiment representative of 2 or more experiments performed in triplicate.