Effects of laminin-8 and laminin-10/11 on SDF-1α–stimulated migration of bone marrow progenitor cells across Transwell inserts.

(A) Migration of CD34⁺ cells across Transwell inserts coated with PBS, laminin-8 (LN-8), or laminin-10/11 (LN-10/11). Shown are means ± SDs of 1 experiment representative of 2 experiments performed in triplicate. (B,C) Inhibition of migration of CD34⁺ cells across Transwell inserts coated with laminin-8 (B) and laminin-10/11 (C) by monoclonal antibody GoH3 against integrin α6 chain. Migration was studied in the absence of antibodies (no ab), in the presence of the GoH3 antibody against integrin α6 (int.α6), and in the presence of irrelevant rat monoclonal antibody (rIgG). Asterisks indicate a significant difference from the control value (migration across Transwell inserts coated with PBS in panel A; migration in the absence of antibodies in panels B and C). ***P < .001.