Fig. 2.
Localization of TRPC1 and TRPC6 in highly purified platelet membranes.
Shown are Western blot analyses of platelet mixed membranes (MM) prior to high-voltage free flow electrophoresis (FFE) and purified plasma (PM) and intracellular membranes (IM) prepared using FFE. (A) 100 μg protein was probed with anti–XTRP-1 (1 μg/mL) and (B) 50 μg protein was probed with anti-TRPC6 (1/400). Molecular size markers are noted in kilodaltons (kDa) and the TRPC protein indicated with arrows.