Fig. 6.
![Fig. 6. Serine or threonine phosphorylation of TRPC1 and TRPC6. / [32P]Pi-labeled platelets were used in a resting state (R) or incubated with 2 U/mL thrombin (T), 500 μM BIMPS (B), or 500 μM 8PCPT (8P). (A) Autoradiography (of dried-down gel) using 50 μL platelet lysates per lane. (B) Autoradiography of Ank immunoprecipitation blot. (C) Autoradiography of anti-TRPC6 immunoprecipitation blot. In (B) and (C), lower panels represent immune detection of blots with Ank and anti-TRPC6, respectively. Molecular size markers are noted in kilodaltons (kDa), TRPC and coimmunoprecipitated phosphoproteins are indicated with arrows.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/100/8/10.1182_blood-2002-03-0723/4/h82023238006.jpeg?Expires=1735527230&Signature=FfQL11wJMmW8nENmyCvazux3zWgqdHyakBNYRucQbKafFlohgR2UOiRX-HRgmp0IO6pHX9TgiHYvYc1z8uf6r2i4bE7zJ0HTR3L8WQ6HAl6pjtz-o3zhe1an4B9BFprRDIFdACBuiAuKk5PBAov6cCQ8LO~4j0YnKudcoy21GE843uhRW~nw1uvIaEfG37aCndBgKAl0OdGfP8lyW94oXoDGicqN38bkfqx9yhaD53HWPM8vx9C6Xpt4G9LK-mMhLV-UdrnNIgFvtcG6ks3lPU~tnebpuT2fBnIlNWbIWOk1kSbDkxRa4-DHqnDC~OVU4fu8-Y0ZakIt2N3wTFzyPQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Serine or threonine phosphorylation of TRPC1 and TRPC6.
[32P]Pi-labeled platelets were used in a resting state (R) or incubated with 2 U/mL thrombin (T), 500 μM BIMPS (B), or 500 μM 8PCPT (8P). (A) Autoradiography (of dried-down gel) using 50 μL platelet lysates per lane. (B) Autoradiography of Ank immunoprecipitation blot. (C) Autoradiography of anti-TRPC6 immunoprecipitation blot. In (B) and (C), lower panels represent immune detection of blots with Ank and anti-TRPC6, respectively. Molecular size markers are noted in kilodaltons (kDa), TRPC and coimmunoprecipitated phosphoproteins are indicated with arrows.
