SU5416 and SU5614 inhibition of proliferation of BaF3 FLT3 ITD-mutant cells but not parental BaF3 cells.

Cells were exposed to inhibitor for 72 hours, and proliferation was assessed with an XTT-based assay. Proliferation is expressed as a percentage of vehicle-treated control cells (error bars indicate standard deviation). (A) In BaF3 FLT3 ITD-mutant cells, SU5416 and SU5614 had the most potent inhibitory effects on proliferation. The IC₅₀ for SU5416 was 250 nM while SU5614 inhibited proliferation at all concentrations tested. By comparison, SU6597 and SU6668 only inhibited proliferation at a 5 μM dose. (B) The proliferation of parental BaF3 cells grown in the presence of IL-3 was not significantly affected by any of the tested concentrations.